Angiotensin-converting enzyme in Spodoptera littoralis: Molecular characterization, expression and activity profile during development

Els Lemeire, Bartel Vanholme, Thomas Van Leeuwen, John Van Camp, Guy Smagghe

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

Uittreksel

The characterization of the full-length angiotensin-converting enzyme (ACE) cDNA sequence of the lepidopteran Spodoptera littoralis is reported in this study. The predicted open reading frame encodes a 647 amino acids long protein (SlACE) and shows 63.6% identity with the Bombyx mori ACE sequence. A 3D-model, consisting of 26 a-helices and three b-sheets, was predicted for the sequence. SlACE expression was studied in the embryonic, larval and pupal stages of S. littoralis and in different tissues of the last larval stage by reversetranscribed PCR. This revealed that the gene is expressed throughout the life cycle and especially in brain, gut and fat body tissue of the last stage. These results are in agreement with a role of ACE in the metabolism of neuropeptides and gut hormones. In addition, ACE activity has been studied in more detail during development, making use of a fluorescent assay. High ACE peptidase activity coincides with every transition state, from embryo to larva, from larva to larva and from larva to pupa. A peak value in activity occurs during the early pupal stage. These results indicate the importance of SlACE during metamorphosis and reveal the high correlation of ACE activity
with the insect’s development, which is regulated by growth and developmental hormones. r 2007 Elsevier Ltd. All rights reserved.
TaalEngels
TijdschriftInsect Biochemistry and Molecular Biology
Volume38
Pagina's (van-tot)166–175
Aantal pagina's10
ISSN0965-1748
StatusGepubliceerd - 2008
Extern gepubliceerdJa

Trefwoorden

  • B410-landbouwhydrologie

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