Application of GISH to characterise Buddleja and Hibiscus hybrids

Katrijn Van Laere, Ludmila Khrustaleva, Johan Van Huylenbroeck, Erik Van Bockstaele

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureC1: Artikels in proceedings van wetenschappelijke congressen, die niet inbegrepen zijn in A1, A2, A3 of P1peer review

Uittreksel

Genomic In Situ Hybridization (GISH) is a powerful technique for detecting parental origin of interspecific hybrids and monitoring alien chromatin introgressed in breeding lines. In this study, successful use of GISH for woody ornamentals, characterized by a small genome and high numbers of small-sized chromosomes, is demonstrated. GISH was performed on Buddleja and Hibiscus hybrids resulting from an interspecific breeding program between different species of both genera. Using B. globosa as a probe, GISH analyses on Buddleja ×weyeriana (a F2 of B. globosa × B. davidii) and F1 and F2 hybrids of B. davidii × B. ×weyeriana crosses proved that all chromatin material of B. globosa was introgressed into the B. davidii chromosomes. Also F2 hybrids of H. syriacus × H. paramutabilis were analysed using GISH with H. syriacus as a probe. Also here recombinant chromosomes showing introgression of H. syriacus DNA in H. paramutabilis were detected.
Oorspronkelijke taalEngels
TitelProceedings of the First International Symposium on Woody Ornamentals of the Temperate Zone
EditorsF Sramek, J Dostalkova, S Chaloupkova
Aantal pagina’s6
Volume885
Publicatiedatum2010
Pagina's411-416
ISBN van geprinte versie978-90-6605-663-3
PublicatiestatusGepubliceerd - 2010

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