TY - JOUR
T1 - Assessment of the suitability of mannitol salt agar for growing bovine-associated coagulase-negative staphylococci and its use under field conditions
AU - De Visscher, A
AU - Haesebrouck, F
AU - Piepers, S
AU - Vanderhaeghen, W
AU - Supré, K
AU - Leroy, F
AU - Van Coillie, Els
AU - De Vliegher, S
N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.
PY - 2013
Y1 - 2013
N2 - This study aimed at testing the applicability of mannitol salt agar (MSA), a medium generally used in human medicine for differentiating Staphylococcus aureus from coagulase-negative staphylococci (CNS), for culturing bovine-associated CNS species. All test isolates from a comprehensive collection of well-identified CNS species, including both reference strains and field isolates, were able to grow. Subsequently, bulk milk samples and teat apex swabs were used to examine the capability of MSA for yielding CNS under field conditions. Sixty-nine and 47 phenotypically different colonies were retrieved from bulk milk and teat apices, respectively. The majority of isolates from teat apices were staphylococci, whereas in bulk milk, staphylococci formed a minority. After 24h of growth, recovery of separate colonies of CNS was much more convenient on MSA compared to a non-selective blood agar. The results of this study indicate that MSA is a suitable medium for both growth and recovery of bovine-associated CNS.
AB - This study aimed at testing the applicability of mannitol salt agar (MSA), a medium generally used in human medicine for differentiating Staphylococcus aureus from coagulase-negative staphylococci (CNS), for culturing bovine-associated CNS species. All test isolates from a comprehensive collection of well-identified CNS species, including both reference strains and field isolates, were able to grow. Subsequently, bulk milk samples and teat apex swabs were used to examine the capability of MSA for yielding CNS under field conditions. Sixty-nine and 47 phenotypically different colonies were retrieved from bulk milk and teat apices, respectively. The majority of isolates from teat apices were staphylococci, whereas in bulk milk, staphylococci formed a minority. After 24h of growth, recovery of separate colonies of CNS was much more convenient on MSA compared to a non-selective blood agar. The results of this study indicate that MSA is a suitable medium for both growth and recovery of bovine-associated CNS.
U2 - 10.1016/j.rvsc.2013.05.015
DO - 10.1016/j.rvsc.2013.05.015
M3 - A1: Web of Science-article
C2 - 23809647
SN - 0034-5288
VL - 95
SP - 347
EP - 351
JO - Research in Veterinary Science
JF - Research in Veterinary Science
IS - 2
ER -