Uittreksel
In this article we describe the isolation and
characterisation of the junction between insert DNA and
plant DNA in the transgenic Roundup Ready soybean
line event 40-3-2. Our results establish that during integration
of the insert DNA several rearrangements occurred
at the 3' NOS junction and that the genomic plant
DNA at the pre-integration site may have been rearranged.
These findings highlight the utility of characterising
junction regions to fulfil the request for information
regarding which DNA sequences have been incorporated
in commercialised transgenic lines. Furthermore,
the characterisation of junction regions is, in our opinion,
the method of choice to support method development
for detection and identification of plant biotechnology-
derived products.
characterisation of the junction between insert DNA and
plant DNA in the transgenic Roundup Ready soybean
line event 40-3-2. Our results establish that during integration
of the insert DNA several rearrangements occurred
at the 3' NOS junction and that the genomic plant
DNA at the pre-integration site may have been rearranged.
These findings highlight the utility of characterising
junction regions to fulfil the request for information
regarding which DNA sequences have been incorporated
in commercialised transgenic lines. Furthermore,
the characterisation of junction regions is, in our opinion,
the method of choice to support method development
for detection and identification of plant biotechnology-
derived products.
| Oorspronkelijke taal | Engels |
|---|---|
| Tijdschrift | European Food Research and Technology |
| Volume | 213 |
| Pagina's (van-tot) | 107-112 |
| Aantal pagina’s | 6 |
| Publicatiestatus | Gepubliceerd - 2001 |