TY - JOUR
T1 - Characterization in biological traits of entomopathogenic nematodes isolated from North China
AU - Ma, Juan
AU - Chen, Shulong
AU - Moens, Maurice
AU - De Clercq, Patrick
AU - Li, Xiuhua
AU - Han, Richou
N1 - Copyright © 2013 Elsevier Inc. All rights reserved.
PY - 2013
Y1 - 2013
N2 - In a series of bioassays, thirty-one isolates that were collected from diverse locations in northern China and the laboratory kept isolate Steinernema carpocapsae All, were compared in order to select superior isolates for biological control of Bradysia odoriphaga. Virulence of the isolates against B. odoriphaga was significantly different among nematode isolates. Tolerance of infective juveniles (IJs) to heat, cold, and desiccation differed significantly among and within species. Strains from S. carpocapsae, S. ceratophorum, S. longicaudum, Heterorhabditis indica, and H. bacteriophora were more heat tolerant than strains from S. feltiae, S. hebeiense, S. monticolum, and H. megidis. Heterorhabditis megidis, H. bacteriophora, and S. carpocapsae showed better cold tolerance than the other species. High desiccation tolerance was recorded for S. carpocapsae, S. hebeiense, and S. ceratophorum. The infectivity of IJ of these species against Galleria mellonella larvae was not significantly different between the treated and non-treated IJ after the nematodes had been exposed to 40°C for 2h, -5°C for 8h or 25% glycerin for 72h. Nematode survival was significantly affected by exposure time and IJ concentration when exposed to 40°C or -5°C. All nematode isolates lost their infectivity against G. mellonella after exposure to -5°C for 16h, except for H. megidis LFS10, which had a low infectivity of 3.3%. A hierarchical classification analysis classified the isolates in four main clusters. The fourth cluster, composed of 13 isolates, grouped the isolates that scored well for most traits.
AB - In a series of bioassays, thirty-one isolates that were collected from diverse locations in northern China and the laboratory kept isolate Steinernema carpocapsae All, were compared in order to select superior isolates for biological control of Bradysia odoriphaga. Virulence of the isolates against B. odoriphaga was significantly different among nematode isolates. Tolerance of infective juveniles (IJs) to heat, cold, and desiccation differed significantly among and within species. Strains from S. carpocapsae, S. ceratophorum, S. longicaudum, Heterorhabditis indica, and H. bacteriophora were more heat tolerant than strains from S. feltiae, S. hebeiense, S. monticolum, and H. megidis. Heterorhabditis megidis, H. bacteriophora, and S. carpocapsae showed better cold tolerance than the other species. High desiccation tolerance was recorded for S. carpocapsae, S. hebeiense, and S. ceratophorum. The infectivity of IJ of these species against Galleria mellonella larvae was not significantly different between the treated and non-treated IJ after the nematodes had been exposed to 40°C for 2h, -5°C for 8h or 25% glycerin for 72h. Nematode survival was significantly affected by exposure time and IJ concentration when exposed to 40°C or -5°C. All nematode isolates lost their infectivity against G. mellonella after exposure to -5°C for 16h, except for H. megidis LFS10, which had a low infectivity of 3.3%. A hierarchical classification analysis classified the isolates in four main clusters. The fourth cluster, composed of 13 isolates, grouped the isolates that scored well for most traits.
U2 - 10.1016/j.jip.2013.08.012
DO - 10.1016/j.jip.2013.08.012
M3 - A2: International peer reviewed article (not A1-type)
C2 - 24035764
SN - 0022-2011
VL - 114
SP - 268
EP - 276
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
IS - 3
ER -