Combined high-performance liquid chromatography and radioimmunoassay for the screening of 19-nortestosterone and methyltestosterone residues in meat samples

E Daeseleire, A De Guesquière, C Van Peteghem

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    A procedure is described for the detection of 17 beta-19-nortestosterone (17 beta-19-NT) and 17 alpha-methyltestosterone (17 alpha-MT) in muscle tissue by a combination of high-performance liquid chromatography and radioimmunoassay. The steroids were released from the muscle tissue by enzyme digestion, and the extracts were purified by solid-phase extraction. A meat sample from the retail trade, which was proved to contain 17 beta-19-NT and 17 alpha-MT residues by gas chromatography-mass spectrometry, and blank meat samples obtained from non-treated experimental animals were purified and the extracts injected into the liquid chromatograph. With an automatic fraction collector, fifteen fractions of 1 ml each were obtained, which after evaporation were subjected to a radioimmunoassay for the steroid concerned. It was demonstrated that none of the fractions from the blank meat samples contains any substance that interfered with the immunochemical detection system. In addition, a good qualitative agreement between the two sets of results was obtained. Although the sample preparation step is rather labour-intensive, the method can be successfully applied as a reliable confirmation method for positive radioimmunoassay screening results in circumstances where no gas chromatography-mass spectrometry facilities are available.
    TaalEngels
    TijdschriftJournal of Chromatography A
    Volume564
    Exemplaarnummer2
    Pagina's (van-tot)445-9
    Aantal pagina's5
    ISSN0021-9673
    StatusGepubliceerd - 1991

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