Comparative genomics in the boxwood blight system: Insights into the global diversity of the mating-type locus

M. Malapi-Wight, J. Hébert, Y. Rivera, E. Ismaiel, N. Saied, Bjorn Gehesquière, Kurt Heungens, Jo-Anne Crouch

    Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureC3: Congres abstract

    Uittreksel

    The ascomycete Calonectria pseudonaviculata, a destructive fungal pathogen
    responsible for boxwood blight disease, was first reported in the U.S. in 2011.
    In a matter of months, North American boxwood blight outbreaks occurred
    across the eastern seaboard and Pacific Northwest. In order to understand the
    sexual reproductive potential of C. pseudonaviculata, we have applied next
    generation sequencing to assess the primary determinant of mating (MAT1)
    locus. The genome of fifteen C. pseudonaviculata isolates were sequenced,
    used to create a local BLAST databases in CLC Genomics, and then searched
    for homologues of the idiomorphic genes MAT1-1 and MAT1-2. Only a single
    MAT1 idiomorph was identified in each of the fifteen genome assemblies,
    indicating that C. pseudonaviculata is a heterothallic fungus. Subsequently,
    we designed specific primers to amplify differently sized regions for each
    MAT1 locus. Over 200 isolates originating from four continents were analyzed
    by a duplexed PCR reaction. PCR analyses revealed that the mating types
    correspond with the genetic lineages G1 and G2. MAT1-2 genotype is globally
    distributed, but exclusively in the G1 lineage. The MAT1-1 genotype is solely
    present in five European countries from isolates of the G2 lineage. These
    results indicate that the fungus is currently unable to sexually reproduce in
    North America, since all the U.S. isolates belonged to the MAT1-2 genotype.
    TaalEngels
    TitelPhytopathology
    Aantal pagina's1
    Volume104
    UitgeverijAmerican Phytopathological Society (APS)
    Datumnov-2014
    Uitgave11, S3
    Pagina's74
    StatusGepubliceerd - nov-2014
    EvenementAnnual APS Conference - Minneapolis, Verenigde Staten van Amerika
    Duur: 9-aug-201413-aug-2014

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