Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes

Jo Bijttebier; Kelly Tilleman; Maarten Dhaenens; Dieter Deforce; Ann Van Soom; Dominiek Maes

doi:10.1002/pmic.200900270

Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes

Jo Bijttebier, Kelly Tilleman, Maarten Dhaenens, Dieter Deforce, Ann Van Soom, Dominiek Maes

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Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous serum and pFF was performed to investigate proteins involved in successful cumulus expansion of porcine oocytes. iTRAQ labeling followed by 2-D LC ESI-Q-TOF MS/MS revealed 63 proteins common to both fluids of which the abundance of 13 proteins was significantly different (p

Oorspronkelijke taal	Engels
Tijdschrift	Proteomics
Volume	9
Exemplaarnummer	19
Pagina's (van-tot)	4554-65
Aantal pagina’s	12
DOI's	https://doi.org/10.1002/pmic.200900270
Publicatiestatus	Gepubliceerd - 2009

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10.1002/pmic.200900270

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@article{51149e81f0d9433ca90ea85529906f99,

title = "Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes",

abstract = "Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous serum and pFF was performed to investigate proteins involved in successful cumulus expansion of porcine oocytes. iTRAQ labeling followed by 2-D LC ESI-Q-TOF MS/MS revealed 63 proteins common to both fluids of which the abundance of 13 proteins was significantly different (p",

keywords = "Animals, Blood Proteins, Cumulus Cells, Female, Follicular Fluid, Humans, Molecular Weight, Oocytes, Protein Array Analysis, Proteome, Swine, alpha-Macroglobulins",

author = "Jo Bijttebier and Kelly Tilleman and Maarten Dhaenens and Dieter Deforce and {Van Soom}, Ann and Dominiek Maes",

year = "2009",

doi = "10.1002/pmic.200900270",

language = "English",

volume = "9",

pages = "4554--65",

journal = "Proteomics",

issn = "1615-9853",

publisher = "Wiley-VCH Verlag",

number = "19",

}

Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes. / Bijttebier, Jo; Tilleman, Kelly; Dhaenens, Maarten; Deforce, Dieter; Van Soom, Ann; Maes, Dominiek.

In: Proteomics, Vol. 9, Nr. 19, 2009, blz. 4554-65.

Onderzoeksoutput: Bijdrage aan tijdschrift › A1: Web of Science-artikel

TY - JOUR

T1 - Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes

AU - Bijttebier, Jo

AU - Tilleman, Kelly

AU - Dhaenens, Maarten

AU - Deforce, Dieter

AU - Van Soom, Ann

AU - Maes, Dominiek

PY - 2009

Y1 - 2009

N2 - Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous serum and pFF was performed to investigate proteins involved in successful cumulus expansion of porcine oocytes. iTRAQ labeling followed by 2-D LC ESI-Q-TOF MS/MS revealed 63 proteins common to both fluids of which the abundance of 13 proteins was significantly different (p

AB - Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous serum and pFF was performed to investigate proteins involved in successful cumulus expansion of porcine oocytes. iTRAQ labeling followed by 2-D LC ESI-Q-TOF MS/MS revealed 63 proteins common to both fluids of which the abundance of 13 proteins was significantly different (p

KW - Animals

KW - Blood Proteins

KW - Cumulus Cells

KW - Female

KW - Follicular Fluid

KW - Humans

KW - Molecular Weight

KW - Oocytes

KW - Protein Array Analysis

KW - Proteome

KW - Swine

KW - alpha-Macroglobulins

U2 - 10.1002/pmic.200900270

DO - 10.1002/pmic.200900270

M3 - A1: Web of Science-article

C2 - 19688730

VL - 9

SP - 4554

EP - 4565

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 19

ER -

Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha(2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes

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