Detection of airborne genetically modified maize pollen by real-time PCR

Silvia Folloni, Dafni-Maria Kagkli, Bojan Rajcevic, Nilson C C Guimarães, Bart Van Droogenbroeck, Fernando H Valicente, Guy VAN den Eede, Marc VAN den Bulcke

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review

    Uittreksel

    The cultivation of genetically modified (GM) crops has raised numerous concerns in the European Union and other parts of the world about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dispersal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GM maize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semi-urban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GM pollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this 'GM pollen monitoring by bioaerosol sampling and PCR screening' approach might represent an useful aid in the surveillance of GM-free areas, centres of origin and natural reserves.
    Oorspronkelijke taalEngels
    TijdschriftMolecular Ecology Resources
    Volume12
    Exemplaarnummer5
    Pagina's (van-tot)810-821
    Aantal pagina’s12
    DOI's
    PublicatiestatusGepubliceerd - 2012

    Trefwoorden

    • T490-biotechnologie
    • GMO detection
    • coexistence

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