Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

Leen Baert, Christiane E Wobus, Els Van Coillie, Larissa B Thackray, Johan Debevere, Mieke Uyttendaele

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.
    TaalEngels
    TijdschriftApplied and environmental microbiology
    Volume74
    Exemplaarnummer2
    Pagina's (van-tot)543-6
    Aantal pagina's4
    ISSN0099-2240
    DOI's
    StatusGepubliceerd - 2008

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