Determination of halofuginone in eggs by liquid chromatography/tandem mass spectrometry after cleanup with immunoaffinity chromatography

L Mortier, E Daeseleire, A-C Huet, P Delahaut, C Van Peteghem

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    A sensitive and very selective high-performance liquid chromatography/tandem mass spectrometric (LC/MS/MS) method for the detection of halofuginone in whole egg has been developed. After deproteinisation with acetonitrile and evaporation of the organic solvent, halofuginone was further isolated by applying immunoaffinity chromatography. The concentrated eluent was injected into the LC/MS/MS system on a C18 column. The precursor ion ([M+H]+) produced by positive electrospray ionisation was selected for fragmentation with argon. Validation parameters such as recovery, linearity and repeatability, decision limit (CCalpha) and detection capability (CCbeta) were determined.
    TaalEngels
    TijdschriftRapid Communications in Mass Spectrometry
    Volume18
    Exemplaarnummer16
    Pagina's (van-tot)1817-20
    Aantal pagina's4
    ISSN0951-4198
    DOI's
    StatusGepubliceerd - 2004

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