Development and validation of a multi-residue LC-MS/MS analysis of non-steroidal anti-inflammatory drugs in milk and meat

    Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureC1: Artikels in proceedings van wetenschappelijke congressen, die niet inbegrepen zijn in A1, A2, A3 of P1


    Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used substances in veterinary medicine for treating fever, pain and inflammation (e.g., coliform mastitis) in food-producing animals. Their widespread administration and occurrence in animal-derived products (meat and milk), impose a potential risk for humans. Maximum residue limits (MRLs) have been set for some (Commission Regulation 37/2010), implying the need for sensitive analytical methods to detect, identify and quantify possible residues.
    This study aimed to develop and validate a multi-residue method for milk and meat to simultaneously detect, identify and quantify NSAIDs by liquid chromatography-tandem mass spectrometry (LC-MS/MS): carprofen, diclofenac, phenylbutazone, flufenamic acid, flunixin (or marker metabolite 5-hydroxy-flunixin in milk), ketoprofen, 4-methylaminoantipyrine (marker metabolite of metamizole in milk and meat), meloxicam and tolfenamic acid.
    Mass spectrometric parameters and chromatographic conditions were optimized on a MicroMass Quattro Ultima triple quadrupole (Waters) and the Acquity UPLC (Waters) system, respectively. H2O/MeCN (95/5) + 0.1 % formic acid (solvent A) and MeCN + 0.1 % formic acid (solvent B) were used for chromatographic separation. Extraction was performed using MeCN. These methods were validated according to Commission Decision 2002/657/EC. Performance characteristics evaluated were specificity, linearity, recovery, repeatability, intra-laboratory reproducibility, decision limit and detection capability.
    Oorspronkelijke taalEngels
    TitelProceedings of Euroresidue VII
    EditorsRobert Schilt
    Aantal pagina’s7
    ISBN van geprinte versie978-94-6173-318-4
    PublicatiestatusGepubliceerd - 14-mei-2012
    EvenementEuroResidue VII Conference on Residues of Veterinary Drugs in Food - Egmond aan Zee, Egmond aan Zee, Nederland
    Duur: 14-mei-201216-mei-2012


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