TY - JOUR
T1 - Discovery based high resolution MS/MS analysis for selection of allergen markers in chocolate and broth powder matrices
AU - Pilolli, Rosa
AU - Van Poucke, Christof
AU - De Angelis, Elisabetta
AU - Nitride, Chiara
AU - de Loose, Marc
AU - Gillard, Nathalie
AU - Huet, Anne Catherine
AU - Tranquet, Olivier
AU - Larré, Colette
AU - Adel-Patient, Karine
AU - Bernard, Hervé
AU - Mills, E. N.Clare
AU - Monaci, Linda
PY - 2020
Y1 - 2020
N2 - Peptide marker identification is an important step in development of a mass spectrometry method for multiple allergen detection, since specificity, robustness and sensitivity of the overall analytical method will depend on the reliability of the proteotypic peptides. As part of the development of a multi-analyte reference method, discovery analysis of two incurred food matrices has been undertaken to select the most reliable peptide markers. Six allergenic ingredients (milk, egg, peanut, soybean, hazelnut, and almond) were incurred into either chocolate or broth powder matrix. Different conditions of protein extraction and purification were tested and the tryptic peptide pools were analysed by untargeted high resolution tandem mass spectrometry and the resulting fragmentation spectra were processed via a commercial software for sequence identification. The analysis performed on incurred foods provides both a prototype effective and straightforward sample preparation protocol and delivers reliable peptides to be included in a standardized selected reaction monitoring method.
AB - Peptide marker identification is an important step in development of a mass spectrometry method for multiple allergen detection, since specificity, robustness and sensitivity of the overall analytical method will depend on the reliability of the proteotypic peptides. As part of the development of a multi-analyte reference method, discovery analysis of two incurred food matrices has been undertaken to select the most reliable peptide markers. Six allergenic ingredients (milk, egg, peanut, soybean, hazelnut, and almond) were incurred into either chocolate or broth powder matrix. Different conditions of protein extraction and purification were tested and the tryptic peptide pools were analysed by untargeted high resolution tandem mass spectrometry and the resulting fragmentation spectra were processed via a commercial software for sequence identification. The analysis performed on incurred foods provides both a prototype effective and straightforward sample preparation protocol and delivers reliable peptides to be included in a standardized selected reaction monitoring method.
KW - Discovery analysis
KW - Food allergens
KW - Incurred matrix
KW - Mass spectrometry
KW - Peptide markers
KW - ThRAll
UR - https://www.mendeley.com/catalogue/da6b3dfa-454a-3b5f-9255-1497ae629c73/
U2 - 10.1016/j.foodchem.2020.128533
DO - 10.1016/j.foodchem.2020.128533
M3 - Article
JO - FOOD CHEMISTRY
JF - FOOD CHEMISTRY
SN - 0308-8146
ER -