Eco-, geno- and human toxicology of bio-active nanoparticles for biomedical applications

Johan Robbens, Caroline Vanparys, Ingrid Nobels, Ronny Blust, Karen Van Hoecke, Colin Janssen, Karel De Schamphelaere, Kathleen Roland, Gersande Blanchard, Frédéric Silvestre, Virginie Gillardin, Patrick Kestemont, Roel Anthonissen, Olivier Toussaint, Sébastien Vankoningsloo, Christelle Saout, Ernesto Alfaro-Moreno, Peter Hoet, Laetitia Gonzalez, Peter DubruelPaul Troisfontaines

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    Gene delivery has become an increasingly important strategy for treating a variety of human diseases, including infections, genetic disorders and tumours. To avoid the difficulties of using viral carriers, more and more non-viral gene delivery nanoparticles are developed. Among these new approaches polyethylene imine (PEI) is currently considered as one of the most effective polymer based method solution and considered as the gold standard. The toxicity of nanoparticles is a major concern when used for medical application. In this study we chose two nanoparticles for an in depth toxicological and ecotoxicological evaluation, one well characterized, PEI, and another novel polymer, poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA). In the present study we have assessed the toxicity of these cation nanoparticles as such and of the polyplexes - nanoparticles covered with DNA. As these nanoparticles are also frequently used in high volumes in various industries and as such may enter in the environment, we also made an initial assessment of ecotoxicological effects assessment. The following nanoparticles related aspects have been studied during the project: development and characterization, ecotoxicity, general toxicity and specific toxicity. To this end a battery of different tests was used. The conclusion of these tests is that toxicity is varying between different nanoparticles and between different DNA covering ratios. In general, in the different systems tested, the PEI polymer is more toxic than the PDMAEMA polymer. The same difference is seen for the polyplexes and the higher the charge ratio, the more toxic are the polyplexes. Our study also clearly shows the need for a broad spectrum of toxicity assays for a comprehensive risk assessment. Our study has performed such a comprehensive analysis of two biomedical nanoparticles.
    TaalEngels
    TijdschriftArchives of Toxicology
    Volume269
    Exemplaarnummer2-3
    Pagina's (van-tot)170-81
    Aantal pagina's12
    ISSN0340-5761
    DOI's
    StatusGepubliceerd - 2010

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