Een vernieuwende en geïntegreerde benadering op basis van DNA walking voor identificatie van niet-geautoriseerde GGO's

Marie-Alice Fraiture, Philippe Herman, Isabel Taverniers, Marc De Loose, Dieter Deforce, Nancy H Roosens

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

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    In the coming years, the frequency of unauthorised genetically modified organisms (GMOs) being present in the European food and feed chain will increase significantly. Therefore, we have developed a strategy to identify unauthorised GMOs containing a pCAMBIA family vector, frequently present in transgenic plants. This integrated approach is performed in two successive steps on Bt rice grains. First, the potential presence of unauthorised GMOs is assessed by the qPCR SYBR Green technology targeting the terminator 35S pCAMBIA element. Second, its presence is confirmed via the characterisation of the junction between the transgenic cassette and the rice genome. To this end, a DNA walking strategy is applied using a first reverse primer followed by two semi-nested PCR rounds using primers that are each time nested to the previous reverse primer. This approach allows to rapidly identify the transgene flanking region and can easily be implemented by the enforcement laboratories.
    TaalEngels
    TijdschriftFOOD CHEMISTRY
    Volume147
    Pagina's (van-tot)60-69
    Aantal pagina's10
    ISSN0308-8146
    DOI's
    StatusGepubliceerd - 2014

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