Effect of Ace Inhibitors and TMOF on Growth, Development, and Trypsin Activity of Larval Spodoptera littoralis

Angiotensin converting enzyme (ACE) is a zinc metallopeptidase capable of cleaving dipeptide or dipeptideamide moieties at the
C-terminal end of peptides. ACE is present in the hemolymph and reproductive tissues of insects. The presence of ACE in the
hemolymph and its broad substrate specificity suggests an important role in processing of bioactive peptides. This study reports
the effects of ACE inhibitors on larval growth in the cotton leafworm Spodoptera littoralis. Feeding ACE inhibitors ad lib decreased
the growth rate, inhibited ACE activity in the larval hemolymph, and down-regulated trypsin activity in the larval gut. These
results indicate that S. littoralis ACE may influence trypsin biosynthesis in the larval gut by interacting with a trypsin-modulating
oostatic factor (TMOF). Injecting third instar larvae with a combination of Aea-TMOF and the ACE inhibitor captopril, downregulated
trypsin biosynthesis in the larval gut indicating that an Aea-TMOF gut receptor analogue could be present. Injecting
captopril and enalapril into newly molted fifth instar larvae stopped larval feeding and decreased weight gain. Together, these
results indicate that ACE inhibitors are efficacious in stunting larval growth and ACE plays an important role in larval growth and
development. Arch Insect Biochem Physiol.