Effect of biochar amendment on the rhizosphere microbiome of lettuce and strawberry

Jane Debode, Caroline De Tender, Pieter Cremelie, Bart Vandecasteele, Martine Maes

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureC3: Congres abstract


Biochar, a solid coproduct of biomass pyrolysis, can be used as soil amendment. It thereby has potential to help mitigate climate change, as it permanently sequesters carbon from the atmosphere and it may be beneficial for agricultural crops. We investigate the effects of biochar soil amendment on plant health and rhizosphere microbiology (FP7-Fertiplus project).
For 2-3 months, lettuce was grown in field soil and strawberry was grown in peat, each amended with 0, 1 or 3% biochar produced from holm oak. Changes in chemical soil/substrate and plant properties were measured. The associated changes in the rhizosphere microbiome were studied using phospholipid fatty acid (PLFA) analysis and amplicon sequencing of the bacterial V3-V4 region of the 16SrDNA.
Biochar amendment had a different effect on the lettuce-soil versus the strawberry-peat rhizosphere. For the lettuce-soil rhizosphere, PLFA analysis showed that the 3% biochar application increased the population of arbuscular mycorrhizal fungi, whereas no significant shifts could be observed in the bacterial microbiome based on both PLFA and 16S analysis. For the strawberry-peat rhizosphere, PLFA analysis showed a significant clustering of the 3% biochar application as compared to the 0% and 1% applications. This was confirmed by the 16S analysis, which dedicated the shift to the reduction in relative abundance of the Proteobacteria, mainly Burkholderia species. This microbial information was combined with plant and substrate properties, showing a relationship between strawberry rhizobiome, biochar application rate, plant growth, plant resistance to Botrytis cinerea and substrate moisture and water-soluble phosphorus content.
In sum, PLFA analysis and 16S amplicon sequencing showed that biochar incorporation can have an influence on the rhizosphere microbiome, associated with induced plant growth and health. This influence was more pronounced in the peat substrate than in soil, which is a more diverse and complex environment and is thus probably less sensitive to disturbance.
Oorspronkelijke taalEngels
TitelAbstract book Rhizosphere 4
PublicatiestatusGepubliceerd - 2015
EvenementRhizosphere 4 - Maastricht, Nederland
Duur: 21-jun-201525-jun-2015

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