Effect of grape seed extract on human norovirus GII.4 and murine norovirus 1 in viral suspensions, on stainless steel discs, and in lettuce wash water

Dan Li, Leen Baert, Dongsheng Zhang, Ming Xia, Weiming Zhong, Els Van Coillie, Xi Jiang, Mieke Uyttendaele

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

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    The anti-norovirus (anti-NoV) effect of grape seed extract (GSE) was examined by plaque assay for murine norovirus 1 (MNV-1), cell-binding reverse transcription-PCR for human NoV GII.4, and saliva-binding enzyme-linked immunosorbent assay for human NoV GII.4 P particles, with or without the presence of interfering substances (dried milk and lettuce extract). GSE at 0.2 and 2 mg/ml was shown to reduce the infectivity of MNV-1 (>3-log PFU/ml) and the specific binding ability of NoV GII.4 to Caco-2 cells (>1-log genomic copies/ml), as well as of its P particles to salivary human histo-blood group antigen receptors (optical density at 450 nm of >0.8). These effects were decreased as increasing concentrations of dried milk (0.02 and 0.2%) or lettuce extract were added. Under an electron microscope, human NoV GII.4 virus-like particles showed inflation and deformation after treatment with GSE. Under conditions that simulated applications in the food industry, the anti-NoV effect of GSE using MNV-1 as a target organism was shown to be limited in surface disinfection (
    TaalEngels
    TijdschriftApplied and environmental microbiology
    Volume78
    Exemplaarnummer21
    Pagina's (van-tot)7572-8
    Aantal pagina's7
    ISSN0099-2240
    DOI's
    StatusGepubliceerd - 2012

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