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A series of growth trials with broiler chicks was conducted in various geographical locations to evaluate the efficacy of a novel eubiotic feed additive (EFA) at various dietary inclusion levels on performance of growing chicks. The EPA product consisted of a blend of essential oil compounds (thymol, eugenol, piperine) with benzoic acid, all belonging to the group of flavoring substances. Although variable in responses, the overall results indicated that 300 mg/kg of this EFA represented an optimum supplementation dose for generation of beneficial performance effects in broilers. A meta-analysis with all data from the 300 mg/kg EFA-supplemented treatments in comparison with the non-supplemented controls revealed that the eubiotic product significantly improved BW on d 21 (+2.0 P = 0.0021) and on d 42 (+1.4 P = 0.0151). Furthermore, the birds on the EPA 300 mg/kg treatment expressed a higher average daily gain in the starter phase (d 1-21; +2.1 P = 0.0023) and over the entire experimental period (d 1-42; +1.5 P = 0.0154). Feed conversion ratio was more favorable with dietary EFA supplementation (-0.6 P = 0.0414), when compared with the control birds. Mortality was considered normal and was not affected by the dietary treatment (control = 3.09 EFA 300 mg/kg = 3.26. In conclusion, 300 mg/kg of this new eubiotic product demonstrated to effectively improve performance of broiler chicks under various husbandry conditions.
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