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Interspecific crosses within Hibiscus resulted in F1 and F2 (self-pollination of F1) progeny of H. syriacus x H. paramutabilis. Of these populations seven F1 and five F2 hybrids were analysed for the occurrence of unreduced gametes. Pollen from H. syriacus, H. paramutabilis, and the F1 and F2 hybrids had diameters between 80 - 230 µm. However, two of the F2 hybrids tested also had a few pollen (<#1%) with a diameter of 250 µm. Flow cytometry and a study of meiosis in the pollen of these two F2 hybrids revealed that 6 - 10% unreduced pollen was produced. Both the parent plants, H. syriacus ‘Oiseau Bleu’ and H. paramutabilis, and all the F1 hybrids were tetraploid with a genome size between 2.32 - 2.48 pg 1C-1. However, all five F2 hybrids were hexaploid, with a genome size between 3.49 - 3.59 pg 1C-1. Since no unreduced pollen could be detected in the F1 H. syriacus x H. paramutabilis hybrids, either by flow cytometry or by a study of meiosis in the pollen, it could be concluded that the F1 hybrids must produce unreduced egg cells.
|Tijdschrift||Journal of Horticultural Science & Biotechnology|
|Publicatiestatus||Gepubliceerd - 2009|
VingerafdrukBekijk de onderzoeksthema's van 'Evidence for the occurrence of unreduced gametes in interspecific hybrids of Hibiscus'. Samen vormen ze een unieke vingerafdruk.
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