Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

Bruno Studer, Torben Asp, Ursula Frei, Stephan Hentrup, Helena Meally, Aurelie Guillard, Susanne Barth, Hilde Muylle, Isabel Roldan-Ruiz, Philippe Barre, Carole Koning-Boucoiran, Gerda Uenk-Stunnenberg, Oene Dolstra, Leif Skot, Kirsten P. Skot, Lesley B. Turner, Mervyn O. Humphreys, Roland Kolliker, Niels Roulund, Klaus K. NielsenThomas Lubberstedt

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel


    An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.
    TijdschriftMolecular Breeding
    Pagina's (van-tot)533-548
    Aantal pagina's16
    StatusGepubliceerd - 2008


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