Projecten per jaar
Uittreksel
Detection of food-borne viruses such as noroviruses, rotaviruses and hepatitis A virus in food products differs from detection of most food-borne bacteria, as most of these viruses cannot be cultivated in cell culture to date. Therefore, detection of food-borne viruses in food products requires multiple steps: first, virus extraction; second, purification of the viral genomic material (RNA for the majority of food-borne viruses); and last, molecular detection. This review is focused on the first step, the virus extraction. All of the numerous published protocols for virus extraction from food samples are based on 3 main approaches: 1) (acid adsorption-) elution-concentration; 2) direct RNA extraction; and 3) proteinase K treatment. This review summarizes these virus extraction approaches and the results obtained from published protocols. The use of process controls is also briefly described.
Oorspronkelijke taal | Engels |
---|---|
Tijdschrift | International journal of food microbiology |
Volume | 153 |
Exemplaarnummer | 1-2 |
Pagina's (van-tot) | 1-9 |
Aantal pagina’s | 9 |
ISSN | 0168-1605 |
DOI's | |
Publicatiestatus | Gepubliceerd - 2012 |
Vingerafdruk
Bekijk de onderzoeksthema's van 'Extraction of food-borne viruses from food samples: a review'. Samen vormen ze een unieke vingerafdruk.Projecten
- 1 Afgerond
-
TRAVIFOOD: Onderzoek naar de virussen NoV, HAV en HEV die overdraagbaar zijn via bepaalde voedingsmiddelen en bijdrage aan de risicobeoordeling
Van Coillie, E. (Projectverantwoordelijke) & Stals, A. (Voormalig Onderzoeker)
1/01/11 → 31/12/13
Project: Onderzoek