First isolation of hepatitis E virus genotype 4 in Europe through swine surveillance in the Netherlands and Belgium

Renate W Hakze-van der Honing; Els Van Coillie; Adriaan F G Antonis; Wim H M van der Poel

doi:10.1371/journal.pone.0022673

First isolation of hepatitis E virus genotype 4 in Europe through swine surveillance in the Netherlands and Belgium

Renate W Hakze-van der Honing, Els Van Coillie, Adriaan F G Antonis, Wim H M van der Poel

Onderzoeksoutput: Bijdrage aan tijdschrift › A1: Web of Science-artikel › peer review

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Hepatitis E virus (HEV) genotypes 3 and 4 are a cause of human hepatitis and swine are considered the main reservoir. To study the HEV prevalence and characterize circulating HEV strains, fecal samples from swine in the Netherlands and Belgium were tested by RT-PCR. HEV prevalence in swine was 7-15%. The Dutch strains were characterized as genotype 3, subgroups 3a, 3c and 3f, closely related to sequences found in humans and swine earlier. The HEV strains found in Belgium belonged to genotypes 3f and 4b. The HEV genotype 4 strain was the first ever reported in swine in Europe and an experimental infection in pigs was performed to isolate the virus. The genotype 4 strain readily infected piglets and caused fever and virus shedding. Since HEV4 infections have been reported to run a more severe clinical course in humans this observation may have public health implications.

Oorspronkelijke taal	Engels
Tijdschrift	PloS one
Volume	6
Exemplaarnummer	8
Pagina's (van-tot)	e22673
DOI's	https://doi.org/10.1371/journal.pone.0022673
Publicatiestatus	Gepubliceerd - 2011

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10.1371/journal.pone.0022673

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@article{70155919d05440289dcf26e910564745,

title = "First isolation of hepatitis E virus genotype 4 in Europe through swine surveillance in the Netherlands and Belgium",

abstract = "Hepatitis E virus (HEV) genotypes 3 and 4 are a cause of human hepatitis and swine are considered the main reservoir. To study the HEV prevalence and characterize circulating HEV strains, fecal samples from swine in the Netherlands and Belgium were tested by RT-PCR. HEV prevalence in swine was 7-15%. The Dutch strains were characterized as genotype 3, subgroups 3a, 3c and 3f, closely related to sequences found in humans and swine earlier. The HEV strains found in Belgium belonged to genotypes 3f and 4b. The HEV genotype 4 strain was the first ever reported in swine in Europe and an experimental infection in pigs was performed to isolate the virus. The genotype 4 strain readily infected piglets and caused fever and virus shedding. Since HEV4 infections have been reported to run a more severe clinical course in humans this observation may have public health implications.",

keywords = "Animals, Base Sequence, Belgium, DNA Primers, Enzyme-Linked Immunosorbent Assay, Genotype, Hepatitis E, Hepatitis E virus, Humans, Netherlands, Open Reading Frames, Phylogeny, Real-Time Polymerase Chain Reaction, Swine",

author = "{Hakze-van der Honing}, {Renate W} and {Van Coillie}, Els and Antonis, {Adriaan F G} and {van der Poel}, {Wim H M}",

year = "2011",

doi = "10.1371/journal.pone.0022673",

language = "English",

volume = "6",

pages = "e22673",

journal = "PloS one",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "8",

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T1 - First isolation of hepatitis E virus genotype 4 in Europe through swine surveillance in the Netherlands and Belgium

AU - Hakze-van der Honing, Renate W

AU - Van Coillie, Els

AU - Antonis, Adriaan F G

AU - van der Poel, Wim H M

PY - 2011

Y1 - 2011

N2 - Hepatitis E virus (HEV) genotypes 3 and 4 are a cause of human hepatitis and swine are considered the main reservoir. To study the HEV prevalence and characterize circulating HEV strains, fecal samples from swine in the Netherlands and Belgium were tested by RT-PCR. HEV prevalence in swine was 7-15%. The Dutch strains were characterized as genotype 3, subgroups 3a, 3c and 3f, closely related to sequences found in humans and swine earlier. The HEV strains found in Belgium belonged to genotypes 3f and 4b. The HEV genotype 4 strain was the first ever reported in swine in Europe and an experimental infection in pigs was performed to isolate the virus. The genotype 4 strain readily infected piglets and caused fever and virus shedding. Since HEV4 infections have been reported to run a more severe clinical course in humans this observation may have public health implications.

AB - Hepatitis E virus (HEV) genotypes 3 and 4 are a cause of human hepatitis and swine are considered the main reservoir. To study the HEV prevalence and characterize circulating HEV strains, fecal samples from swine in the Netherlands and Belgium were tested by RT-PCR. HEV prevalence in swine was 7-15%. The Dutch strains were characterized as genotype 3, subgroups 3a, 3c and 3f, closely related to sequences found in humans and swine earlier. The HEV strains found in Belgium belonged to genotypes 3f and 4b. The HEV genotype 4 strain was the first ever reported in swine in Europe and an experimental infection in pigs was performed to isolate the virus. The genotype 4 strain readily infected piglets and caused fever and virus shedding. Since HEV4 infections have been reported to run a more severe clinical course in humans this observation may have public health implications.

KW - Animals

KW - Base Sequence

KW - Belgium

KW - DNA Primers

KW - Enzyme-Linked Immunosorbent Assay

KW - Genotype

KW - Hepatitis E

KW - Hepatitis E virus

KW - Humans

KW - Netherlands

KW - Open Reading Frames

KW - Phylogeny

KW - Real-Time Polymerase Chain Reaction

KW - Swine

U2 - 10.1371/journal.pone.0022673

DO - 10.1371/journal.pone.0022673

M3 - A1: Web of Science-article

C2 - 21829641

SN - 1932-6203

VL - 6

SP - e22673

JO - PloS one

JF - PloS one

IS - 8

ER -

First isolation of hepatitis E virus genotype 4 in Europe through swine surveillance in the Netherlands and Belgium

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