Flow cytometric analysis of ploidy in Rhododendron (subgenus Tsutsusi)

Sandra De Schepper, Leen Leus, Marleen Mertens, Pierre Debergh, Jozef Heursel, Erik Van Bockstaele, Marc De Loose

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

Uittreksel

Ploidy level was determined for six species and 88 cultivars of the Rhododendron subgenus Tsutsusi, High-resolution flow cytometry of nuclear DNA was performed on macerated plant tissue. All plants analyzed were diploid (2n = 26) with the exception of 'Euratom, 'Euratom Orange', and 'Red Wing', which were triploid (3n = 39), and 'Casablanca Tetra', which was found to be a cytochimera: mixoploid (2n + 4n) in the LI and LII, but tetraploid in the LIII. The described method has proven to be useful in screening a large population of rhododendrons, Analysis of different organs and plant tissues was easily accomplished through flow cytometry, and has proven useful in determining the ploidy df different histogenic layers.
TaalEngels
TijdschriftHortscience
Volume36
Pagina's (van-tot)125-127
ISSN0018-5345
StatusGepubliceerd - 2001

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