Flow cytometric detection of unreduced pollen in Begonia

A. Dewitte, Tom Eeckhaut, J. Van Huylenbroeck, E. Van Bockstaele

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureC1: Artikels in proceedings van wetenschappelijke congressen, die niet inbegrepen zijn in A1, A2, A3 of P1

Uittreksel

Unreduced gametes, formed due to a disruption in meiosis, are considered to play an important role in the formation of polyploids in nature. A screening method for the detection of unreduced gametes in ornamentals is however needed. Begonia was used as a model plant for the detection of unreduced pollen. Chemical, enzymatic, mechanical and osmotic assays have been used for the isolation of nuclei from Begonia pollen. The relative DNA content of pollen nuclei was measured using flow cytometry. Two methods, on germinated and non-germinated pollen, were successfully applied. Flow cytometric analysis showed two peaks in pollen, associated with the vegetative (1C) and generative (2C) nuclei of normal (reduced) pollen. Two of seven tested Begonia genotypes showed a peak at the 4C level, associated with the presence of unreduced gametes. Formation of unreduced gametes was associated with abnormalities in pollen shape or pollen size.
TaalEngels
TitelProceedings of the 22nd International Eucarpia Symposium Section Ornamentals: Breeding for Beauty
EditorsT Schiva
Aantal pagina's6
Volume714
Datum2006
Pagina's107-112
ISBN van geprinte versie978-90-66055-49-0
StatusGepubliceerd - 2006

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