Housekeeping gene selection for Real Time-PCR normalization in female Hop (Humulus lupulus L) tissues

Lina Maloukh, Jaroslav Matousek, Erik Van Bockstaele, Isabel Roldan-Ruiz

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review

Uittreksel

The variability of transcript accumulation of six genes encoding chloropyll-a/b (Chla/b) binding protein, Nicotinamide Adenine Dinucleotide Hydride (NADH) dehydrogenase, Histone H3 (H3); DEAD-box RNA helicase 1 (DRH1) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and of the 7SL component of the. signal recognition particle (7SL-RNA), was estimated in leaves and female inflorescences of the two hop cultivars, White Golding and Admiral at different developmental stages by RT-PCR. The value of these genes as internal, normalization controls in gene transcript accumulation studies was assessed. The combination of the three housekeeping genes DRH1, GAPDH and 7SL-RNA as internal standards for hop, provided reliable results in the quantitative analysis of the transcript, accumulation of Hua Enhancer 1 transcription factor (HEN1) homologue in hop tissues and is recommended for future studies of gene expression in female hop tissues.

Oorspronkelijke taalEngels
TijdschriftJournal of Plant Biochemistry and Biotechnology
Volume18
Exemplaarnummer1
Pagina's (van-tot)53-58
Aantal pagina’s6
PublicatiestatusGepubliceerd - 2009

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