Identification and substrate prediction of new Fragaria x ananassa aquaporins andexpression in different tissues and during strawberry fruit development

Britt Merlaen, Ellen De Keyser, Marie-Christine Van Labeke

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review

    Uittreksel

    The newly identified aquaporin coding sequences presented here pave the way for further insights into the plant–water relations in the commercial strawberry (Fragaria x ananassa). Aquaporins are water channel proteins that allow water to cross (intra)cellular membranes. In Fragaria x ananassa, few of them have been identified hitherto, hampering the exploration of the water transport regulation at cellular level. Here, we present new aquaporin coding sequences belonging to different subclasses: plasma membrane intrinsic proteins subtype 1 and subtype 2 (PIP1 and PIP2) and tonoplast intrinsic proteins (TIP). The classification is based on phylogenetic analysis and is confirmed by the presence of conserved residues. Substrate-specific signature sequences (SSSSs) and specificity-determining positions (SDPs) predict the substrate specificity of each new aquaporin. Expression profiling in leaves, petioles and developing fruits reveals distinct patterns, even within the same (sub)class. Expression profiles range from leaf-specific expression over constitutive expression to fruit-specific expression. Both upregulation and downregulation during fruit ripening occur. Substrate specificity and expression profiles suggest that functional specialization exists among aquaporins belonging to a different but also to the same (sub)class.
    Oorspronkelijke taalEngels
    Artikel nummer20
    TijdschriftHorticulture Research
    Volume5
    Exemplaarnummer1
    Pagina's (van-tot)20
    ISSN2052-7276
    DOI's
    PublicatiestatusGepubliceerd - 2018

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