Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum

Annelies Vercauteren; Meg Larsen; Erica Goss; Niklaus J Grünwald; Martine Maes; Kurt Heungens

doi:10.3852/10-420

Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum

Annelies Vercauteren, Meg Larsen, Erica Goss, Niklaus J Grünwald, Martine Maes, Kurt Heungens

Onderzoeksoutput: Bijdrage aan tijdschrift › A1: Web of Science-artikel › peer review

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Phytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the limited intralineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphic microsatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified and as such can help better characterize the North American lineages of P. ramorum.

Oorspronkelijke taal	Engels
Tijdschrift	Mycologia
Volume	103
Exemplaarnummer	6
Pagina's (van-tot)	1245-9
Aantal pagina’s	5
ISSN	0027-5514
DOI's	https://doi.org/10.3852/10-420
Publicatiestatus	Gepubliceerd - 2011

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@article{feb6ee1fb9ba4f6eb824ff85d3f38ea9,

title = "Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum",

abstract = "Phytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the limited intralineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphic microsatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified and as such can help better characterize the North American lineages of P. ramorum.",

keywords = "Base Sequence, Genetic Variation, Genotype, Microsatellite Repeats, Molecular Sequence Data, Phytophthora, Polymorphism, Genetic",

author = "Annelies Vercauteren and Meg Larsen and Erica Goss and Gr{\"u}nwald, {Niklaus J} and Martine Maes and Kurt Heungens",

year = "2011",

doi = "10.3852/10-420",

language = "English",

volume = "103",

pages = "1245--9",

journal = "Mycologia",

issn = "0027-5514",

publisher = "Allen Press Inc.",

number = "6",

}

TY - JOUR

T1 - Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum

AU - Vercauteren, Annelies

AU - Larsen, Meg

AU - Goss, Erica

AU - Grünwald, Niklaus J

AU - Maes, Martine

AU - Heungens, Kurt

PY - 2011

Y1 - 2011

N2 - Phytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the limited intralineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphic microsatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified and as such can help better characterize the North American lineages of P. ramorum.

AB - Phytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the limited intralineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphic microsatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified and as such can help better characterize the North American lineages of P. ramorum.

KW - Base Sequence

KW - Genetic Variation

KW - Genotype

KW - Microsatellite Repeats

KW - Molecular Sequence Data

KW - Phytophthora

KW - Polymorphism, Genetic

U2 - 10.3852/10-420

DO - 10.3852/10-420

M3 - A1: Web of Science-article

C2 - 21642345

SN - 0027-5514

VL - 103

SP - 1245

EP - 1249

JO - Mycologia

JF - Mycologia

IS - 6

ER -

Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum

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