In vitro polyploidy induction in Rhododendron simsii hybrids

Tom Eeckhaut; G Samyn; E Van Bockstaele

In vitro polyploidy induction in Rhododendron simsii hybrids

Vertaalde titel van de bijdrage: In vitro polyploidy induction in Rhododendron simsii hybrids

Tom Eeckhaut, G Samyn, E Van Bockstaele

Centrale Diensten

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › C1: Artikels in proceedings van wetenschappelijke congressen, die niet inbegrepen zijn in A1, A2, A3 of P1

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In vitro polyploidy induction offers the possibility of quick multiplication over in vivo methods, and is therefore particularly interesting for slowly growing plants. Three different in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was quite labour intensive. Direct sowing on media enriched with antimitotic agents requires less work but is less efficient. To induce ploidy chimeras starting from an existing genotype, mitotic inhibitors can be added to multiplication media.

Vertaalde titel van de bijdrage	In vitro polyploidy induction in Rhododendron simsii hybrids
Oorspronkelijke taal	Engels
Titel	Proceedings of the Twentieth International Eucarpia Symposium - Section Ornamentals: Strategies for New Ornamentals II
Editors	J. Van Huylenbroeck, E Van Bockstaele, P Debergh
Aantal pagina’s	7
Publicatiedatum	2002
Pagina's	43-49
ISBN van geprinte versie	978-90-66058-25-5
Publicatiestatus	Gepubliceerd - 2002

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title = "In vitro polyploidy induction in Rhododendron simsii hybrids",

abstract = "In vitro polyploidy induction offers the possibility of quick multiplication over in vivo methods, and is therefore particularly interesting for slowly growing plants. Three different in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was quite labour intensive. Direct sowing on media enriched with antimitotic agents requires less work but is less efficient. To induce ploidy chimeras starting from an existing genotype, mitotic inhibitors can be added to multiplication media. ",

keywords = "azalea, colchicine, oryzalin, trifluralin, seedlings",

author = "Tom Eeckhaut and G Samyn and {Van Bockstaele}, E",

note = "20th International EUCARPIA Symposium - Section Ornamentals, MELLE, BELGIUM, JUL 03-06, 2001",

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In vitro polyploidy induction in Rhododendron simsii hybrids. / Eeckhaut, Tom; Samyn, G; Van Bockstaele, E.
Proceedings of the Twentieth International Eucarpia Symposium - Section Ornamentals: Strategies for New Ornamentals II. ed. / J. Van Huylenbroeck; E Van Bockstaele; P Debergh. 2002. blz. 43-49 (Acta Horticulturae; Nr. 572).

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/Congresprocedure › C1: Artikels in proceedings van wetenschappelijke congressen, die niet inbegrepen zijn in A1, A2, A3 of P1

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T1 - In vitro polyploidy induction in Rhododendron simsii hybrids

AU - Eeckhaut, Tom

AU - Samyn, G

AU - Van Bockstaele, E

N1 - 20th International EUCARPIA Symposium - Section Ornamentals, MELLE, BELGIUM, JUL 03-06, 2001

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AB - In vitro polyploidy induction offers the possibility of quick multiplication over in vivo methods, and is therefore particularly interesting for slowly growing plants. Three different in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was quite labour intensive. Direct sowing on media enriched with antimitotic agents requires less work but is less efficient. To induce ploidy chimeras starting from an existing genotype, mitotic inhibitors can be added to multiplication media.

KW - azalea

KW - colchicine

KW - oryzalin

KW - trifluralin

KW - seedlings

M3 - C1: Articles in proceedings

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A2 - Van Bockstaele, E

A2 - Debergh, P

ER -

In vitro polyploidy induction in Rhododendron simsii hybrids

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