In vitro propagation of Helleborus species

Emmy Dhooghe, Marie-Christine Van Labeke

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    A general in vitro cloning system was established for four Helleborus species: H. argutifolius, H. foetidus, H. niger and H. orientalis. The plant material was introduced in vitro from axillary buds. A Murashige and Skoog (MS)-based medium (Murashige and Skoog 1962) was used supplemented with 2% (w/v) sucrose, 2-isopentenyladenine (2-iP) and 6-benzylaminopurine (BA). Multiplication rates depended on the genotype and varied from 1.3 for H. foetidus till 3.8 for H. niger. The first results showed that the rooting phase could be done ex vitro. Rooting was induced by a drench for one week in a solution of indole-3-butyric acid (IBA -3 mg l(-1)) and 1-naphthaleneacetic acid (NAA-1 mg l(-1)) at 5 degrees C.
    TaalEngels
    TijdschriftPlant Cell Tissue and Organ Culture
    Volume91
    Exemplaarnummer2
    Pagina's (van-tot)175-177
    Aantal pagina's3
    ISSN0167-6857
    DOI's
    StatusGepubliceerd - 1-nov-2007

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