Induced expression of selected plant defence related genesin pot azalea, Rhododendron simsii hybrid

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    A set of putative marker genes to study
    plant defense responses against Polyphagotarsonemus
    latus, a key pest in the production of Rhododendron
    simsii hybrids, was selected and validated. Genes
    belonged to the biosynthetic pathway of phytohormones
    jasmonic acid (JA) (RsLOX, RsAOS, RsAOC,
    RsOPR3 and RsJMT) and salicylic acid (SA) (RsPAL
    and RsICS). Furthermore, RsPPO, a putative marker
    gene for oxidative stress response was successfully
    cloned from R. simsii. A CTAB-based extraction
    protocol was optimized to assure excellent RNA quality for subsequent RT-qPCR analysis. The RTqPCR
    protocol was extensively tested and RsRG7 and
    RsRG14 were selected as reference genes from a
    geNorm pilot study. Validation of the marker genes
    was done after application with elicitors [methyl
    jasmonate (MeJA), coronatine, b-aminobutyric acid
    and acibenzolar-Smethyl] or wounding. Both 100 lM
    MeJA and 0.1 lM coronatine had a significant effect
    on the expression of all marker genes. Foliar application
    of MeJA on the shoots resulted in a significantly
    earlier response when compared to root application
    and subsequent sampling of the shoots. Expression
    patterns after MeJA treatment were generally the same
    in six R. simsii genotypes: ‘Nordlicht’, ‘Elien’, ‘Aiko
    Pink’, ‘Michelle Marie’, ‘Mevrouw Gerard Kint’ and
    ‘Sachsenstern’. Wounding resulted in the same
    expression patterns as MeJA treatment except for
    RsJMT. None of the genotypes showed a significant
    induction of the latter gene 6 h upon wounding.
    Findings of these experiments indicated that the tolerant genotype ‘Elien’ has low basal expression
    levels of RsPPO. This might be the first step towards
    the breeding of mite-tolerant genotypes.
    TaalEngels
    TijdschriftEuphytica
    Volume213
    Pagina's (van-tot)227
    Aantal pagina's19
    ISSN0014-2336
    DOI's
    StatusGepubliceerd - 2017

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