A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige sr Skoog medium supplemented with 10 mu M naphthaleneacetic acid (NAA) and 0.2 - 3 mu M thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige Skoog (MS) medium supplemented with 10 CIM benzylaminopurine (BA) + 0.02 mu M NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse.
|Titel||Proceedings of the XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding In Vitro Culture|
|Editors||L.H.W. Van der Plas, G.-J. de Klerk|
|ISBN van geprinte versie||978-90-66058-43-9|
|Status||Gepubliceerd - 2000|