Induction of tetraploids in Escallonia spp.

Hanne Denaeghel, Katrijn Van Laere, Leen Leus, Johan Van Huylenbroeck, M.-C. Van Labeke

    Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureC1: Artikels in proceedings van wetenschappelijke congressen, die niet inbegrepen zijn in A1, A2, A3 of P1

    Uittreksel

    Escallonia is a genus of flowering shrubs, native to South-America. As for most woody ornamentals, current breeding efforts in Escallonia are low. In an attempt to enlarge the assortment and to introgress winter hardiness and a more compact growth habit, we performed in vitro polyploidization on Escallonia species. We collected 25 species and characterized their genome size, chromosome number, and leaf and flower parameters. Measured genome sizes of the collection varied between 1.01 and 1.31 pg/2C. In vitro polyploidization on E. rubra and E. rosea was more efficient when using oryzalin and trifluralin compared to colchicine. We obtained up to 56.7% E. rubra tetraploids using trifluralin (1 µM for 6 weeks) and 33.3% E. rosea tetraploids using oryzalin (150 µM for 3 days), compared to colchicine which only yielded 16.7% E. rubra (1000 µM for 3 days) and 3.3% E. rosea (2000 µM for 3 days) tetraploids. Short (2, 3 and 4 days) and chronic (6, 8 and 10 weeks) treatments on E. rubra displayed a higher polyploidization efficiency for trifluralin (28%) compared to oryzalin (18%). Ploidy doubled plantlets and diploid controls were acclimatized in the greenhouse for further morphological evaluation. The most efficient treatment was chosen to polyploidize other Escallonia species.
    TaalEngels
    TitelProceedings of the XXVth International Eucarpia Symposium Section Ornamentals "Crossing Borders"
    EditorsJohan Van Huylenbroeck, Emmy Dhooghe
    Volume1087
    Datum2015
    Pagina's453-458
    ISBN van geprinte versie9789462610781
    StatusGepubliceerd - 2015
    Evenement25th International Eucarpia Symposium Section Ornamentals: Crossing Borders - Melle, België
    Duur: 28-jun-20152-jul-2015

    Vingerafdruk Bekijk de onderzoeksthema's van 'Induction of tetraploids in <em>Escallonia</em> spp.'. Samen vormen ze een unieke vingerafdruk.

    Dit citeren