In floriculture the creation of new cultivars is of major importance. The family of the Ranunculaceae contains some very interesting ornamental plants. Ranunculus asiaticus L and Anemone coronaria L are very common cut flowers. Helleborus orientalis Lam on the other hand has an increasing interest as a perennial garden plant. There is a large amount of variation in leaves, height, flower shape and flower colour within these species. Intergeneric crossings between these genera might therefore result in the creation of new interesting hybrids. Crossing different species and genera might therefore result in the creation of new interesting hybrids. Crossing different species and genera, as e.g. the cross between Ranunculus asiaticus or Anemone coronaria and Helleborus orientalis, is facilitated if chromosome numbers are equal. Helleborus orientalis has a chromosome number of 32 (Zonneveld, 2001) while Ranunculus asiaticus (Goepfert, 1974) and Anemone coronaria (Rothfels et al, 1966) have a chromosome number of 16. Barriers to hybridization may be overcome by manipulating the ploidy levels prior to hybridization. The aim of this study is to double the ploidy level of Ranunculus and Anemone in order to get a better crossing compatibility. In this report we describe the chromosome doubling of Ranunculus asiaticus.
|11th Symposium on Applied Biological Sciences
|Gepubliceerd - 2005