Intraspecific variation in Radopholus similis isolates assessed with restriction fragment length polymorphism and DNA sequencing of the internal transcribed spacer region of the ribosomal RNA cistron.

Gamal A A Elbadri, Paul De Ley, Lieven Waeyenberge, Andy Vierstraete, Maurice Moens, Jacques Vanfleteren

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review

    Uittreksel

    Restriction fragment length polymorphism and direct sequencing of the internal transcribed spacer rDNA region of 19 isolates of Radopholus similis yielded significant diversity, both among isolates and within some individuals. Restriction fragment length polymorphism with HaeIII, AluI and Tru9I yielded two sets of patterns. Digestion with RsaI revealed one or two supernumerary bands in single nematodes from five isolates, and sequencing confirmed microheterogeneity in four of these. Phylogenetic analysis grouped most isolates closely together, except for the five isolates with additional bands for RsaI. Our data reveal more population structure than previously found and lend further support to the synonymy of R. similis and `Radopholus citrophilus'. (C) 2002 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.

    Oorspronkelijke taalEngels
    TijdschriftInternational Journal for Parasitology
    Volume32
    Exemplaarnummer2
    Pagina's (van-tot)199-205
    Aantal pagina’s7
    ISSN0020-7519
    DOI's
    PublicatiestatusGepubliceerd - 2002

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