Different European Biomass Expansion Factors (BEFs) were compared for the inventory-based quantification of total aboveground and belowground biomass in forests. Therefore a qualitative analysis is performed on the biomass results obtained through the BEF approach and those from experimentally established allometric relations based on destructively sampled and fully excavated trees. Total organic carbon (OC) stock in aboveground and belowground living biomass of Flemish forests amounts to 12 Mt on average, with a significantly larger OC stock per hectare in deciduous forests compared to coniferous or mixed forest types. Total forest biomass seems to be fairly well approximated by a multiplication of the standing stock with either one of the applied BEFs. However an indication of the volume and age class for which the BEFs are established and a refined diameter-volume-biomass relation for oak trees in Europe, are required to gain more accurate results.