Invloed van plant ontwikkelingsstadium op DNA opbrengst en extraheerbaarheid in MON810 maïs

Isabel Taverniers, Laurens De Meyer, Bart Van Droogenbroeck, Kathy Messens, Marc De Loose

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    The amount and extractability of DNA in different parts of MON810 GM hybrid was studied during different developmental stages. To quantify GM contents, an evaluation was performed of the effect of plant development stage on DNA density. To this end, the evolution of weights, absolute DNA yields, DNA densities and ratios of endosperm and embryo relative to total maize kernel were studied. Sampling at four stages during the growth shows an influence on relative GM quantification based on haploid genome equivalents, due to the specific maize seed composition and differences in DNA extractability from different seed tissues. During plant growth, plant parts with potential GM genes (embryo in kernel and cob on total plant) increase in importance on weight and DNA concentration level, while the endosperm drops in relative importance. Expected % GM maize values are calculated for a whole field harvest of grain maize.
    TaalEngels
    TijdschriftAgricultural and Food Science
    Volume24
    Pagina's (van-tot)128-138
    Aantal pagina's11
    ISSN1459-6067
    StatusGepubliceerd - 29-jun-2015

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    • B390-plantenteelt

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