Isolation and characterisation of 14 novel microsatellite markers through Next Generation Sequencing for the commercial Atlantic seabob shrimp Xiphopenaeus kroyeri

Thomas R H Kerkhove, Bart Hellemans, Marleen De Troch, Annelies De Backer, Filip A M Volckaert

Onderzoeksoutput: Bijdrage aan tijdschriftArtikel

Uittreksel

Assessing population genetic structure is a crucial step to support fisheries and conservation management. DNA microsatellite molecular markers are a widely used tool in population genotyping. In the present study, we characterised and developed 14 novel polymorphic microsatellite markers for a decapod crustacean, the Atlantic seabob shrimp Xiphopenaeus kroyeri (Heller, 1862), through rapid and cost-effective Illumina shotgun sequencing and a Galaxy-based bioinformatic pipeline. We genotyped 60 individuals from 2 populations with the newly developed microsatellites, resulting in the detection of 3 to 29 alleles per locus. Four loci deviated from Hardy-Weinberg equilibrium. Cross-amplification in a cryptic congeneric species was successful for eight loci (57%). The microsatellite loci developed in this study will be highly relevant for genetic and evolutionary studies of X. kroyeri, and for the stock management of this commercially exploited species.

TaalEngels
TijdschriftMolecular biology reports
Volume46
Exemplaarnummer6
Pagina's (van-tot)6565-6569
Aantal pagina's5
ISSN0301-4851
DOI's
StatusGepubliceerd - dec-2019
Extern gepubliceerdJa

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