Kwantificatie en localisatie van pospiviroïden in bladluizen.

Noémi Van Bogaert, Kris De Jonghe, Els Van Damme, Martine Maes, Guy Smagghe

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    In this paper, the potential role of aphids in viroid transmission was explored. Apterous aphids were fed on pospiviroid-infected plants and viroid targets in the aphids were consequently quantified through RT-qPCR and localized within the aphid body using fluorescence in-situ hybridization (FISH). Based on the analytical sensitivity test, the limit of detection (LOD) was estimated at 1.69*106 viroid copies per individual aphid body. To localize the viroids in the aphids, a pospiviroid-generic Cy5-labelled probe was used and the fluorescent signal was
    determined by confocal microscopy. Viroids were clearly observed in the aphid’s stylet and stomach, but not in the embryos. Viroids were detected in 29% of the aphids after a 24h feeding period, which suggests only a partial and low concentration viroid uptake by the aphid population including viroid concentrations under the LOD. However, these results show that viroids can be ingested by aphids while feeding on infected plants, thus potentially increasing the transmission risk. The combination of FISH and RT-qPCR provides reliable
    and fast localization and quantitation of viroid targets in individual aphids and thus constitutes a valuable tool in future epidemiological research.
    TaalEngels
    TijdschriftJournal of virological methods
    Volume211
    Pagina's (van-tot)51-54
    ISSN0166-0934
    DOI's
    StatusGepubliceerd - jan-2015

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