Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins

Daniël Van Damme, François-Yves Bouget, Kris Van Poucke, Dirk Inzé, Danny Geelen

    Onderzoeksoutput: Bijdrage aan tijdschriftA2: Artikel in een internationaal wetenschappelijk tijdschrift met peer review, dat niet inbegrepen is in A1

    Uittreksel

    To identify molecular players implicated in cytokinesis and division plane determination, the Arabidopsis thaliana genome was explored for potential cytokinesis genes. More than 100 open reading frames were selected based on similarity to yeast and animal cytokinesis genes, cytoskeleton and polarity genes, and Nicotiana tabacum genes showing cell cycle-controlled expression. The subcellular localization of these proteins was determined by means of GFP tagging in tobacco Bright Yellow-2 cells and Arabidopsis plants. Detailed confocal microscopy identified 15 proteins targeted to distinct regions of the phragmoplast and the cell plate. EB1- and MAP65-like proteins were associated with the plus-end, the minus-end, or along the entire length of microtubules. The actin-binding protein myosin, the kinase Aurora, and a novel cell cycle protein designated T22, accumulated preferentially at the midline. EB1 and Aurora, in addition to other regulatory proteins (homologs of Mob1, Sid1, and Sid2), were targeted to the nucleus, suggesting that this organelle operates as a coordinating hub for cytokinesis.

    TaalEngels
    TijdschriftThe Plant journal : for cell and molecular biology
    Volume40
    Exemplaarnummer3
    Pagina's (van-tot)386-98
    Aantal pagina's13
    ISSN0960-7412
    DOI's
    StatusGepubliceerd - 2004

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