Projecten per jaar
detection. This study describes the development of a reliable diagnostic rotocol of LChV-1 based on a one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device and is most optimal when performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the RTLAMP
and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues. In addition, the RT-LAMP has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster (allowing on-field leaf-to-result diagnostic) and efficient at minimal cost. In conclusion, this innovative RT-LAMP approach can contribute to the implementation of sustainable integrated management strategies for detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs.
FOD-EURAVELCH: Unravelling the Little Cherry disease complex at European scale to improve transnational diagnostics and management of the disease
1/05/17 → 30/04/19
IWT-LITTLECHERRY: Kennisgebaseerde praktijkmaatregelen voor een betrouwbare beheersing van Little Cherry
De Jonghe, K. & Tahzima, R.
1/09/15 → 31/08/19