Projecten per jaar
Uittreksel
Little cherry virus 1 (LChV-1) belongs to the genus Velarivirus, family Closteroviridae, is an economically important pathogen affecting mainly cherry around the world emphasizing the impetus for its efficient and accurate on-site
detection. This study describes the development of a reliable diagnostic rotocol of LChV-1 based on a one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device and is most optimal when performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the RTLAMP
and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues. In addition, the RT-LAMP has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster (allowing on-field leaf-to-result diagnostic) and efficient at minimal cost. In conclusion, this innovative RT-LAMP approach can contribute to the implementation of sustainable integrated management strategies for detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs.
detection. This study describes the development of a reliable diagnostic rotocol of LChV-1 based on a one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device and is most optimal when performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the RTLAMP
and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues. In addition, the RT-LAMP has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster (allowing on-field leaf-to-result diagnostic) and efficient at minimal cost. In conclusion, this innovative RT-LAMP approach can contribute to the implementation of sustainable integrated management strategies for detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs.
Taal | Engels |
---|---|
Tijdschrift | Journal of virological methods |
Volume | 265 |
Pagina's (van-tot) | 91-98 |
Aantal pagina's | 8 |
ISSN | 0166-0934 |
DOI's | |
Status | Gepubliceerd - 10-jan-2019 |
Vingerafdruk Bekijk de onderzoeksthema's van 'New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP)'. Samen vormen ze een unieke vingerafdruk.
Projecten
- 2 Afgerond
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FOD-EURAVELCH: Unravelling the Little Cherry disease complex at European scale to improve transnational diagnostics and management of the disease
De Jonghe, K., Haegeman, A. & Tahzima, R.
1/05/17 → 30/04/19
Project: Onderzoek
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IWT-LITTLECHERRY: Kennisgebaseerde praktijkmaatregelen voor een betrouwbare beheersing van Little Cherry
De Jonghe, K. & Tahzima, R.
1/09/15 → 31/08/19
Project: Onderzoek