Non-food/feed seeds as biofactories for the high-yield production of recombinant pharmaceuticals

Francesca Morandini, Linda Avesani, Luisa Bortesi, Bart Van Droogenbroeck, Kirsten De Wilde, Elsa Arcalis, Flavia Bazzoni, Luca Santi, Annalisa Brozzetti, Alberto Falorni, Eva Stoger, Ann Depicker, Mario Pezzotti

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review


    We describe an attractive cloning system for the seed-specific expression of recombinant proteins using three non-food/feed crops. A vector designed for direct subcloning by Gateway® recombination was developed and tested in Arabidopsis, tobacco and petunia plants for the production of a chimeric form (GAD67/65) of the 65 kDa isoform of glutamic acid decarboxylase (GAD65). GAD65 is one of the major human autoantigens involved in type 1 diabetes (T1D). The murine anti-inflammatory cytokine interleukin-10 (IL-10) was expressed with the described system in Arabidopsis and tobacco, whereas proinsulin, another T1D major autoantigen, was expressed in Arabidopsis. The cost-effective production of these proteins in plants could allow the development of T1D prevention strategies based on the induction of immunological tolerance. The best yields were achieved in Arabidopsis seeds, where GAD67/65 reached 7.7% of total soluble protein (TSP), the highest levels ever reported for this protein in plants. IL-10 and proinsulin reached 0.70% and 0.007% of TSP, respectively, consistent with levels previously reported in other plants or tissues. This versatile cloning vector could be suitable for the high-throughput evaluation of expression levels and stability of many valuable and difficult to produce proteins.
    Oorspronkelijke taalEngels
    TijdschriftPlant Biotechnology Journal
    Pagina's (van-tot)911-21
    Aantal pagina’s11
    PublicatiestatusGepubliceerd - 2011


    Bekijk de onderzoeksthema's van 'Non-food/feed seeds as biofactories for the high-yield production of recombinant pharmaceuticals'. Samen vormen ze een unieke vingerafdruk.

    Dit citeren