Phage display as a novel screening tool for primary toxicological targets

Bieke Van Dorst, Wim De Coen, Ronny Blust, Johan Robbens

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    In the present study the use of phage display as a screening tool to determine primary toxicological targets was investigated. These primary toxicological targets are the targets in the cell with which a chemical compound initially interacts and that are responsible for consecutive (toxic) effects. Nickel was used as model compound for the present study. By selection of Ni-binding peptides out of a 12-mer peptide phage library, it was possible to identify primary toxicological targets of Ni (and other metals). The selected Ni-binding peptides showed similarities to important primary toxicological targets of Ni, such as the hydrogenase nickel incorporation protein (hypB) and the Mg/Ni/Co transporter (corA). This shows that phage display, which is already widely used in other research fields, also has potential in ecotoxicology, as a novel screening tool with which to determine primary toxicological targets of chemical compounds.
    TaalEngels
    TijdschriftEnvironmental Toxicology and Chemistry
    Volume29
    Exemplaarnummer2
    Pagina's (van-tot)250-5
    Aantal pagina's6
    ISSN0730-7268
    DOI's
    StatusGepubliceerd - 2010

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