PotatoMASH-A Low Cost, Genome-Scanning Marker System for Use in Potato Genomics and Genetics Applications

Maria de la O. Leyva-Perez, Lea Vexler, Stephen Byrne, Corentin R. Clot, Fergus Meade, Denis Griffin, Tom Ruttink, Jie Kang, Dan Milbourne

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review


We have developed PotatoMASH (Potato Multi-Allele Scanning Haplotags), a novel low-cost, genome-scanning marker platform. We designed a panel of 339 multi-allelic regions placed at 1 Mb intervals throughout the euchromatic portion of the genome. These regions were assayed using a multiplex amplicon sequencing approach, which allows for genotyping hundreds of plants at a cost of 5 EUR/sample. We applied PotatoMASH to a population of over 700 potato lines. We obtained tetraploid dosage calls for 2012 short multi-allelic haplotypes in 334 loci, which ranged from 2 to 14 different haplotypes per locus. The system was able to diagnose the presence of targeted pest-resistance markers, to detect quantitative trait loci (QTLs) by genome-wide association studies (GWAS) in a tetraploid population, and to track variation in a diploid segregating population. PotatoMASH efficiently surveys genetic variation throughout the potato genome, and can be implemented as a single low-cost genotyping platform that will allow the routine and simultaneous application of marker-assisted selection (MAS) and other genotyping applications in commercial potato breeding programmes.
Oorspronkelijke taalEngels
Artikel nummer2461
PublicatiestatusGepubliceerd - 11-okt.-2022


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