Probing genome diversity in natural and breeding populations of Lolium perenne

Tom Ruttink, Elisabeth Veeckman, Annelies Haegeman, Frederik van Parijs, Sabine Van Glabeke, Hilde Muylle, Klaas Vandepoele, Stephen Byrne, Torben Asp, Isabel Roldán-Ruiz

    Onderzoeksoutput: Bijdrage aan congresC3: Congres - Meeting abstractpeer review


    Population-scale genome resequencing has revealed the rich genome sequence diversity in model organisms and agriculturally important crops, and has great potential to identify target loci for breeding and selection or to investigate the genetic basis of adaptive traits. With the dual purpose to provide molecular markers for association mapping and to catalogue the functional sequence variation in the gene pool, we have resequenced several hundred candidate genes in natural accessions, elite breeding material, and commercial varieties of L. perenne. Target loci included key regulatory genes controlling plant growth and development, plant architecture, meristem activity, induction of flowering, light signaling, cell wall biogenesis, and phytohormone biosynthesis, signaling, and response. Based on phylogenetic analysis, we assigned more than 2900 L. perenne proteins predicted on the draft genome to 180 gene families and selected 539 representative family members for targeted resequencing. We constructed indexed shotgun gDNA libraries of 736 independent L. perenne genotypes. These libraries were pooled, probe capture-enriched and sequenced on a HiSeq2000 flowcell. To validate the method, we analysed target region coverage, read depth and polymorphism density, as a function of experimental and SNP calling parameters. Our data provide novel insights into the sequence diversity present in genes and physiological pathways potentially controlling architectural traits and cell wall digestibility, and provide high quality genotyping data for association studies. Furthermore, we aim to predict functional sequence variation based on haplotype reconstruction combined with within- and between-species sequence conservation, and to provide further molecular genetic insight into the causative variation underlying phenotypic diversity.
    Oorspronkelijke taalEngels
    PublicatiestatusGepubliceerd - 10-jan.-2015
    Evenementint. Plant Animal Genome Conference, PAG XXIII - San Diego, Verenigde Staten van Amerika
    Duur: 9-jan.-201514-jan.-2015


    Congresint. Plant Animal Genome Conference, PAG XXIII
    Land/RegioVerenigde Staten van Amerika
    StadSan Diego
    Internet adres


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