Quantification of gene expression of Listeria monocytogenes by real-time reverse transcription PCR: optimization, evaluation and pitfalls

Hadewig Werbrouck, Nadine Botteldoorn, Mieke Uyttendaele, Lieve Herman, Els Van Coillie

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review

Uittreksel

In the current study, various steps in the real-time reverse transcription PCR (real-time RT-PCR) method for determination of RNA expression levels starting from different numbers of Listeria monocytogenes cells were evaluated and optimized. Our results showed that the RNA isolation method as well as the cDNA synthesis may influence the sensitivity of the procedure. For high bacterial cell numbers (10(9) bacterial cells), the RNAqueous kit and the RNeasy Mini kit were equally useful, whereas for low bacterial cell numbers (
Oorspronkelijke taalEngels
TijdschriftJournal of Microbiological Methods
Volume69
Exemplaarnummer2
Pagina's (van-tot)306-314
Aantal pagina’s9
DOI's
PublicatiestatusGepubliceerd - 2007

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