Raman microspectroscopy as an identification tool within the phylogenetically homogeneous 'Bacillus subtilis' group

Didier Hutsebaut, Joachim Vandroemme, Jeroen Heyrman, Peter Dawyndt, Peter Vandenabeele, Luc Moens, Paul de Vos

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

    Uittreksel

    Vibrational methods have multiple advantages compared to more classic, chemotaxonomic and even molecular microbial tools for the identification of bacteria. Nevertheless, their definite breakthrough in diagnostic microbiology laboratories is determined by their identification potential. This paper reports on the profound evaluation of Raman spectroscopy to identify closely related species by means of 68 Bacillus strains that are assigned or closely related to the phylogenetically homogeneous 'Bacillus subtilis'-group (sensu stricto). These strains were chosen to represent biological variation within the selected species and to create a realistic view on the possibilities of this technique The evaluation resulted in 49/54 correct identifications at the species level for intern and 15/19 for extern testing. The correct identification of strains, which were not represented in the training set, supports the potential as an identification tool within the 'B. subtilis group'. Considering the vague borderline between the species studied, Raman spectroscopy can be regarded here as a promising application for identifications at the species level.
    TaalEngels
    TijdschriftSystematic and Applied microbiology
    Volume29
    Exemplaarnummer8
    Pagina's (van-tot)650-60
    Aantal pagina's11
    DOI's
    StatusGepubliceerd - 2006

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