Real-time PCR for the detection of PLMVd and comparison of different nucleic acid extraction protocols.

Martha Luigi; Anna Taglienti; Carla Libia Corrado; Simona  Botti; Rita Bissani; Paola Casati; Niccolo  Miotti; Kris De Jonghe; Ellen Everaert; Antonio Olmos; Ana Ruiz-Garcia; Francesco Faggioli

Real-time PCR for the detection of PLMVd and comparison of different nucleic acid extraction protocols.

Martha Luigi, Anna Taglienti, Carla Libia Corrado, Simona Botti, Rita Bissani, Paola Casati, Niccolo Miotti, Kris De Jonghe, Ellen Everaert, Antonio Olmos, Ana Ruiz-Garcia, Francesco Faggioli

Plant

Onderzoeksoutput: Bijdrage aan congres › Poster

Uittreksel

Peach latent mosaic viroid (PLMVd - genus Pelamoviroid, family Avsunviroidae) is an important pathogen affecting peach (Prunus persica); it is a quarantine pest in many countries (see EPPO and IPPC website). In Europe, visual inspection and testing are required for PLMVd in Prunus persica (Implementing Regulation (EU) 2019/2072). PLMVd symptoms are often variable, unstable and seasonal, and the infection can remain latent for years [1]. Rapid, effective and low-cost diagnostic tests are required for early and reliable PLMVd detection; nonetheless, molecular assay can be impaired due to changes in the rapidly evolving PLMVd genome.
In this work, a duplex reverse transcriptase-real-time PCR (dRT-qPCR) was designed via merging two already published protocols [2,3] to overcome the problem of the high mutation rate. The test was validated according to EPPO Standard PM 7/98. Furthermore, its performance was assessed in a test performance study (TPS) involving five European laboratories.

Oorspronkelijke taal	Engels
Publicatiestatus	Gepubliceerd - 9-jul.-2023
Evenement	25th International Conference on Virus and other fragt transmissible diseasess of fruit crops - WICC in Wageningen, Wageningen, Nederland Duur: 9-jul.-2023 → 13-jul.-2023 https://www.plant-virology.nl/ICVF2023

Congres

Congres	25th International Conference on Virus and other fragt transmissible diseasess of fruit crops
Land/Regio	Nederland
Stad	Wageningen
Periode	9/07/23 → 13/07/23
Internet adres	https://www.plant-virology.nl/ICVF2023

Dit citeren

Luigi, M., Taglienti, A., Corrado, C. L., Botti, S., Bissani, R., Casati, P., Miotti, N., De Jonghe, K., Everaert, E., Olmos, A., Ruiz-Garcia, A., & Faggioli, F. (2023). Real-time PCR for the detection of PLMVd and comparison of different nucleic acid extraction protocols.. Postersessie gepresenteerd op 25th International Conference on Virus and other fragt transmissible diseasess of fruit crops, Wageningen, Nederland.

@conference{4b9eb702e00745398f38fe2f363fd2b8,

title = "Real-time PCR for the detection of PLMVd and comparison of different nucleic acid extraction protocols.",

abstract = "Peach latent mosaic viroid (PLMVd - genus Pelamoviroid, family Avsunviroidae) is an important pathogen affecting peach (Prunus persica); it is a quarantine pest in many countries (see EPPO and IPPC website). In Europe, visual inspection and testing are required for PLMVd in Prunus persica (Implementing Regulation (EU) 2019/2072). PLMVd symptoms are often variable, unstable and seasonal, and the infection can remain latent for years [1]. Rapid, effective and low-cost diagnostic tests are required for early and reliable PLMVd detection; nonetheless, molecular assay can be impaired due to changes in the rapidly evolving PLMVd genome. In this work, a duplex reverse transcriptase-real-time PCR (dRT-qPCR) was designed via merging two already published protocols [2,3] to overcome the problem of the high mutation rate. The test was validated according to EPPO Standard PM 7/98. Furthermore, its performance was assessed in a test performance study (TPS) involving five European laboratories. ",

author = "Martha Luigi and Anna Taglienti and Corrado, {Carla Libia} and Simona Botti and Rita Bissani and Paola Casati and Niccolo Miotti and {De Jonghe}, Kris and Ellen Everaert and Antonio Olmos and Ana Ruiz-Garcia and Francesco Faggioli",

year = "2023",

month = jul,

day = "9",

language = "English",

note = "25th International Conference on Virus and other fragt transmissible diseasess of fruit crops ; Conference date: 09-07-2023 Through 13-07-2023",

url = "https://www.plant-virology.nl/ICVF2023",

}

Luigi, M, Taglienti, A, Corrado, CL, Botti, S, Bissani, R, Casati, P, Miotti, N, De Jonghe, K , Everaert, E, Olmos, A, Ruiz-Garcia, A & Faggioli, F 2023, 'Real-time PCR for the detection of PLMVd and comparison of different nucleic acid extraction protocols.', 25th International Conference on Virus and other fragt transmissible diseasess of fruit crops, Wageningen, Nederland, 9/07/23 - 13/07/23.

TY - CONF

T1 - Real-time PCR for the detection of PLMVd and comparison of different nucleic acid extraction protocols.

AU - Luigi, Martha

AU - Taglienti, Anna

AU - Corrado, Carla Libia

AU - Botti, Simona

AU - Bissani, Rita

AU - Casati, Paola

AU - Miotti, Niccolo

AU - De Jonghe, Kris

AU - Everaert, Ellen

AU - Olmos, Antonio

AU - Ruiz-Garcia, Ana

AU - Faggioli, Francesco

PY - 2023/7/9

Y1 - 2023/7/9

N2 - Peach latent mosaic viroid (PLMVd - genus Pelamoviroid, family Avsunviroidae) is an important pathogen affecting peach (Prunus persica); it is a quarantine pest in many countries (see EPPO and IPPC website). In Europe, visual inspection and testing are required for PLMVd in Prunus persica (Implementing Regulation (EU) 2019/2072). PLMVd symptoms are often variable, unstable and seasonal, and the infection can remain latent for years [1]. Rapid, effective and low-cost diagnostic tests are required for early and reliable PLMVd detection; nonetheless, molecular assay can be impaired due to changes in the rapidly evolving PLMVd genome. In this work, a duplex reverse transcriptase-real-time PCR (dRT-qPCR) was designed via merging two already published protocols [2,3] to overcome the problem of the high mutation rate. The test was validated according to EPPO Standard PM 7/98. Furthermore, its performance was assessed in a test performance study (TPS) involving five European laboratories.

AB - Peach latent mosaic viroid (PLMVd - genus Pelamoviroid, family Avsunviroidae) is an important pathogen affecting peach (Prunus persica); it is a quarantine pest in many countries (see EPPO and IPPC website). In Europe, visual inspection and testing are required for PLMVd in Prunus persica (Implementing Regulation (EU) 2019/2072). PLMVd symptoms are often variable, unstable and seasonal, and the infection can remain latent for years [1]. Rapid, effective and low-cost diagnostic tests are required for early and reliable PLMVd detection; nonetheless, molecular assay can be impaired due to changes in the rapidly evolving PLMVd genome. In this work, a duplex reverse transcriptase-real-time PCR (dRT-qPCR) was designed via merging two already published protocols [2,3] to overcome the problem of the high mutation rate. The test was validated according to EPPO Standard PM 7/98. Furthermore, its performance was assessed in a test performance study (TPS) involving five European laboratories.

M3 - Poster

T2 - 25th International Conference on Virus and other fragt transmissible diseasess of fruit crops

Y2 - 9 July 2023 through 13 July 2023

ER -