TY - JOUR
T1 - Response of DNA fragments to potentiometric sensors studied using HPLC
AU - Nagels, Luc J
AU - Everaert, Joseph
AU - Bohets, Hugo
AU - Del Favero, Jurgen
AU - Goossens, Dirk
AU - Robbens, Johan
AU - Pietraszkiewicz, Marek
AU - Pietraszkiewicz, Oksana
PY - 2007
Y1 - 2007
N2 - Potentiometric sensors are studied as viable candidates for the construction of high throughput DNA arrays. For preliminary investigations, such sensors were used in an HPLC setup in the present work. This avoided errors due to ionic contaminants or additives in the commercial samples. The oligonucleotides dT(10), dT(20) and dT(30) were used as test substances. The potentiometric sensors were of the coated wire type, containing PVC, DOP, MTDDACl and a synthetic podand urea receptor. The HPLC system consisted of a reversed phase column eluted with a phosphate buffer, triethylammoniumacetate (TEAA), and an acetonitrile gradient. Molar responses and sensitivities increased with increasing chain length of oligonucleotides, yielding detection limits as low as 10(-6)M (dT(30), injected concentration). The slopes of the calibration graphs were at least 23 mV/decade (dT(10)), which was much higher than expected. The results are discussed in view of the potential use of this sensor type in high throughput microarrays.
AB - Potentiometric sensors are studied as viable candidates for the construction of high throughput DNA arrays. For preliminary investigations, such sensors were used in an HPLC setup in the present work. This avoided errors due to ionic contaminants or additives in the commercial samples. The oligonucleotides dT(10), dT(20) and dT(30) were used as test substances. The potentiometric sensors were of the coated wire type, containing PVC, DOP, MTDDACl and a synthetic podand urea receptor. The HPLC system consisted of a reversed phase column eluted with a phosphate buffer, triethylammoniumacetate (TEAA), and an acetonitrile gradient. Molar responses and sensitivities increased with increasing chain length of oligonucleotides, yielding detection limits as low as 10(-6)M (dT(30), injected concentration). The slopes of the calibration graphs were at least 23 mV/decade (dT(10)), which was much higher than expected. The results are discussed in view of the potential use of this sensor type in high throughput microarrays.
KW - Calibration
KW - Chromatography, High Pressure Liquid
KW - Electrodes
KW - Molecular Structure
KW - Oligonucleotide Array Sequence Analysis
KW - Oligonucleotides
KW - Potentiometry
KW - Reproducibility of Results
KW - Sensitivity and Specificity
KW - Time Factors
M3 - A1: Web of Science-article
C2 - 17979638
SN - 1386-2073
VL - 10
SP - 555
EP - 559
JO - Combinatorial Chemistry & High Throughput Screening
JF - Combinatorial Chemistry & High Throughput Screening
IS - 7
ER -