Response to strigolactone treatment in chrysanthemum axillary buds is influenced by auxin transport inhibition and sucrose availability

Robrecht Dierck, Emmy Dhooghe, Johan Van Huylenbroeck, Jan De Riek, Ellen De Keyser, Dominique Van Der Straeten

    Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelpeer review


    Axillary bud outgrowth is regulated by both
    environmental cues and internal plant hormone signaling.
    Central to this regulation is the balance between auxins,
    cytokinins, and strigolactones. Auxins are transported
    basipetally and inhibit the axillary bud outgrowth indirectly
    by either restricting auxin export from the axillary buds to
    the stem (canalization model) or inducing strigolactone
    biosynthesis and limiting cytokinin levels (second messenger
    model). Both models have supporting evidence and
    are not mutually exclusive. In this study, we used a modified
    split-plate bioassay to apply different plant growth
    regulators to isolated stem segments of chrysanthemum
    and measure their effect on axillary bud growth. Results
    showed axillary bud outgrowth in the bioassay within
    5 days after nodal stem excision. Treatments with apical
    auxin (IAA) inhibited bud outgrowth which was counteracted
    by treatments with basal cytokinins (TDZ, zeatin,
    2-ip). Treatments with basal strigolactone (GR24) could
    inhibit axillary bud growth without an apical auxin treatment.
    GR24 inhibition of axillary buds could be counteracted
    with auxin transport inhibitors (TIBA and NPA).Treatments with sucrose in the medium resulted in stronger
    axillary bud growth, which could be inhibited with apical
    auxin treatment but not with basal strigolactone treatment.
    These observations provide support for both the canalization
    model and the second messenger model with, on the
    one hand, the influence of auxin transport on strigolactone
    inhibition of axillary buds and, on the other hand, the
    inhibition of axillary bud growth by strigolactone without
    an apical auxin source. The inability of GR24 to inhibit bud
    growth in a sucrose treatment raises an interesting question
    about the role of strigolactone and sucrose in axillary bud
    outgrowth and calls for further investigation.
    Oorspronkelijke taalEngels
    Artikel nummerDOI 10.1007/s11738-016-2292-6
    TijdschriftActa Physiologiae Plantarum
    ExemplaarnummerDOI 10.1007/s11738-016-2292-6
    Pagina's (van-tot)271
    Aantal pagina’s11
    PublicatiestatusGepubliceerd - 2016


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